Nitrate Reductases from Wild-Type and nr1-Mutant Soybean (Glycine max [L.] Merr.) Leaves 1: II. Partial Activity, Inhibitor, and Complementation Analyses

摘要

Soybean (Glycine max [L.] Merr.) leaves have been shown to contain three forms of nitrate reductase (NR). Two of the forms, which are present in leaves of wild-type (cv. Williams) plants grown in the absence of NO3−, are termed constitutive and designated c1NR and c2NR. The third form, which is present in NO3−-grown mutant (nr1) plants lacking the constitutive forms, is termed inducible and designated iNR. Samples of c1NR, c2NR, and iNR obtained from appropriately treated plants were analyzed for the presence of partial activities, response to inhibitors, and ability to complement a barley NR which lacks the molybdenum cofactor (MoCo) but is otherwise active.